CHARACTERIZATION OF THE PROTOPLASMIC CONSTITUENTS OF BACILLUS MEGATERIUM
نویسندگان
چکیده
منابع مشابه
Characterization of the protoplasmic constituents of bacillus megaterium.
It has been shown (Weibull, 1953) that it is possible to isolate protoplasts from Bacillus megaterium by lysozyme treatment of cells suspended in 0.1 to 0.2 M sucrose. When protoplasts are resuspended in phosphate buffer (pH 7), rapid lysis occurs, and the protoplasmic constituents are released. This procedure does not include any mechanical disintegration; in addition, only a small fraction of...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1953
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.66.6.696-702.1953